Using nanoparticles for adsorbing asphaltene was known as one of efficient methods among researchers for upgrading of real oil samples in comparison to other expensive mechanical treatments or even solvents (such as n-pentane and n-heptane) and surfactants. In this study, Nickel zeolite oxide nanoparticles have been used for asphaltene adsorption and solving asphaltene precipitation problems. Although Nickel zeolite oxide nanoparticle used in previous studies as an asphaltene adsorbent, observing relation between asphaltene adsorption on its surface and asphaltene precipitation in the presence of nanoparticles was not covered. Series of experiments include FTIR, CO2-oil IFT tests, Langmuir and Freundlich isotherm models, and natural depletion tests were performed in the presence of Nickel zeolite oxide nanoparticles. Adsorption data was fitted well with the Langmuir model in comparison to the Freundlich model which shows that the adsorption occurs in a homogeneous surface with monolayer coverage. Based on the CO2-oil IFT results, there are two different slope forms in IFT readings as pressure increase from 150 Psi to 1650 Psi. Second slope (900 Psi-1650 Psi) is slower than the first one (150 Psi-900 Psi) which was due to aggregation of asphaltene. Three pressures of 1350 Psi, 1500 Psi, 1650 Psi and Nickel zeolite oxide nanoparticles at concentration of 30 ppm were selected for performing natural depletion tests and the basis of selection was high efficiency of adsorption in these points. As pressure decrease from 1650 Psi to 1350 Psi, asphaltene precipitation changes from 8.25 wt % to 10.52 wt % in the base case and it was 5.17 wt % to 7.54 wt % in the presence of Nickel zeolite oxide 30 ppm. Accordingly, Nickel zeolite oxide nanoparticles adsorbed asphaltene on its surface in proper way and the amount of asphaltene precipitation was decreased in the presence of Nickel zeolite oxide nanoparticles.